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[選]試 譯 文：
(2) Inhibition of fat accumulation
a. Suppresses differentiation of 3T3-L1 adipocytes (in vitro)
Experimental design and treatment:
3T3-L1 adipocytes (5x104 cells/ml) were cul
tured in DMEM medium (high glucose)
supplemented with 10% fetal calf serum. Ce
lls differentiation was induced by medium replacement containing
insulin (1 μg/ml), dexamethasone (0.25
μM) and isobutyl-methyxanthine (0.5 mM). 2 days later, the medium
containing Coffee Bean Extract and its
functional components and insulin (1μg/ml) was introduced.
The new medium was cultured for 6 days
with replacement every alternate day. Differentiation of adipocytes
and cell triglyceride levels were dete
rmined. Cell differentiation was determined using GPDH
(glycerol 3-phosphate dehydrogenase) as biochemical marker.
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