課程名稱︰分子生物學
課程性質︰必修
課程教師︰靳宗洛
開課學院：生命科學院
開課系所︰生命科學系
考試日期（年月日）︰107.11.29
考試時限（分鐘）：100
試題 :
Molecular Biology 29/11/2018
Please answer the following questions succinctly. If you don't know the answer,
it is better just to state so rather than fake it or to give irrelevant
information, Use your limited time wisely.
Term Explanation(5 out of 6; 3% of each)15%
(1) Nick translation.
(2) Primase and integrase.
(3) Glycolase and APE1.
(4) DNA Ligase III and IV.
(5) DNA polymerase α and iDNA.
(6) Mfd, CSA and CSB proteins.
Assay: 15% of each(15%)
(1) Please compare the common component of the priming and replication
complex in Prokaryotic and Eukaryotic cells.
Assay: 10% of each(40%)
(2) Describe the following factors require for eularyotic growth factor (GF)
signal transduction pathway to permit cell cycle progression: RTK, Grb2,
SOS, RAS, RAF, MEK, ERK, scaffolding protein CNK and KSR, Rb/E2F, cyclin
/CDK complex.
(3) How FstZ, SlmA, and MinCDE control septum formation and localization.
(4) Double strand DNA break recombination in E. coli involves with (a) Rec
complexes, (b) chi site and (c) Ruv factors, please describe the
mechanism.
(5) Describe the NER action of the best known UvrABC excinuclease in E. coli
and XPs in Eukaryotic system.
Choice 3 from the following questions, 10% of each(30%)
(6) Describe the bacteria two-component signaling system, and how the vir
genes functioning in T-DNA transfer from Agrobacteria to plant??
(7) How to trigger SOS response and how to repair the DNA during the
stress??
(8) How to control the ColE1 plasmid replication, and P1 and R1 plasmid
segregation.
(9) Describe the mismatch repair(MMR) system in Bacteria cells, and the
conserved non-homologous end joining(NHEJ) mechanism.
(10) How to deal with the oxidized Guanine for repairing in Eukaryotic
cells??
(11) Describe the factors, ORC, Cdc6, Cdt1 and MCM, require for yeast DNA
replication, and the most recently knowkedge on MCM activation.
(12) 3'-OH is essential for DNA replication/elongation, please describe 3
dofferent ways to provide the 3'-OH.
(13) How to stabilize the end length of chromosome????